The file titled sequences.fasta contains several DNA sequences relevant to a Golden Gate assembly project…
The file titled sequences.fasta contains several DNA sequences relevant to a Golden Gate assembly project…: a task in Task Alignment Bench (TAB) (Harbor dataset). I want to use the NEBridge Golden Gate assembly kit with BsaI-HF v2 enzyme to assemble fragments together into the desired output…
The task
I want to use the NEBridge Golden Gate assembly kit with BsaI-HF v2 enzyme to assemble fragments together into the desired output. However, I don't have enzyme cut-sites in my sequences so I'll need to PCR amplify them first.
Part of anonymous98570239854/tab.